Overview

At Moffitt, The Binning Lab will apply cutting-edge biochemical and structural techniques to identify new pathogenic mechanisms employed by oncogenic human papilloma viruses (HPV) that are drivers of cervical and head and neck cancers. The fundamental work that will be pursued in Dr. Binning’s laboratory will inform the development of diagnostics and therapeutics for these cancers.

Associations

Gynecologic Oncology
Molecular Oncology
Cancer Biology & Evolution Program

Education & Training

Graduate:

Iowa State University, PhD - Biochemistry

Research Interest

The Binning lab studies how viruses such as Human Papillomavirus (HPV) re-wire host ubiquitin machinery to support viral replication and drive tumorigenesis. By exploring the link between human disease states and dysregulation of ubiquitin pathways, we aim to identify new pathogenic mechanisms that will guide the development of diagnostics and therapeutics.

Publications

Welsch JT, Smalley TB, Matlack JK, Avalon NE, Binning JM, Johnson MP, Allcock AL, Baker BJ. Tuaimenals B-H, Merosesquiterpenes from the Irish Deep-Sea Soft Coral Duva florida with Bioactivity against Cervical Cancer Cell Lines. J Nat Prod. 2023 Jan.86(1):182-190. Pubmedid: 36580354. Pmcid: PMC9887596.
Avalon NE, Nafie J, De Marco Verissimo C, Warrensford LC, Dietrick SG, Pittman AR, Young RM, Kearns FL, Smalley T, Binning JM, Dalton JP, Johnson MP, Woodcock HL, Allcock AL, Baker BJ. Tuaimenal A, a Meroterpene from the Irish Deep-Sea Soft Coral Duva florida, Displays Inhibition of the SARS-CoV-2 3CLpro Enzyme. J Nat Prod. 2022 May.85(5):1315-1323. Pubmedid: 35549259. Pmcid: PMC9127705.
Wang Y, Binning JM, Pintilie GD, Chiu W, Amarasinghe GK, Leung DW, Su Z. Cryo-EM analysis of Ebola virus nucleocapsid-like assembly. STAR Protoc. 2022 Mar.3(1):101030. Pubmedid: 34977676. Pmcid: PMC8689349.
Bekker RA, Zahid MU, Binning JM, Spring BQ, Hwu P, Pilon-Thomas S, Enderling H. Rethinking the immunotherapy numbers game. J Immunother Cancer. 2022 Jul.10(7). Pubmedid: 35793871. Pmcid: PMC9260835.
Binning JM, Chesarino NM, Emerman M, Gross JD. Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism. Cell Host Microbe. 2019 Dec.26(6):739-747.e4. Pubmedid: 31830442. Pmcid: PMC6913891.
Ball KA, Chan LM, Stanley DJ, Tierney E, Thapa S, Ta HM, Burton L, Binning JM, Jacobson MP, Gross JD. Conformational Dynamics of the HIV-Vif Protein Complex. Biophys J. 2019 Apr.116(8):1432-1445. Pubmedid: 30961890. Pmcid: PMC6486493.
Binning JM, Smith AM, Hultquist JF, Craik CS, Caretta Cartozo N, Campbell MG, Burton L, La Greca F, McGregor MJ, Ta HM, Bartholomeeusen K, Peterlin BM, Krogan NJ, Sevillano N, Cheng Y, Gross JD. Fab-based inhibitors reveal ubiquitin independent functions for HIV Vif neutralization of APOBEC3 restriction factors. PLoS Pathog. 2018 Jan.14(1):e1006830. Pubmedid: 29304101. Pmcid: PMC5773222.
Su Z, Wu C, Shi L, Luthra P, Pintilie GD, Johnson B, Porter JR, Ge P, Chen M, Liu G, Frederick TE, Binning JM, Bowman GR, Zhou ZH, Basler CF, Gross ML, Leung DW, Chiu W, Amarasinghe GK. Electron Cryo-microscopy Structure of Ebola Virus Nucleoprotein Reveals a Mechanism for Nucleocapsid-like Assembly. Cell. 2018 Feb.172(5):966-978.e12. Pubmedid: 29474922. Pmcid: PMC5973842.
Faust TB, Binning JM, Gross JD, Frankel AD. Making Sense of Multifunctional Proteins: Human Immunodeficiency Virus Type 1 Accessory and Regulatory Proteins and Connections to Transcription. Annu Rev Virol. 2017 Sep.4(1):241-260. Pubmedid: 28961413. Pmcid: PMC5750048.
Kane JR, Stanley DJ, Hultquist JF, Johnson JR, Mietrach N, Binning JM, Jónsson SR, Barelier S, Newton BW, Johnson TL, Franks-Skiba KE, Li M, Brown WL, Gunnarsson HI, Adalbjornsdóttir A, Fraser JS, Harris RS, Andrésdóttir V, Gross JD, Krogan NJ. Lineage-Specific Viral Hijacking of Non-canonical E3 Ubiquitin Ligase Cofactors in the Evolution of Vif Anti-APOBEC3 Activity. Cell Rep. 2015 May.11(8):1236-1250. Pubmedid: 25981045. Pmcid: PMC4613747.
Leung DW, Borek D, Luthra P, Binning JM, Anantpadma M, Liu G, Harvey IB, Su Z, Endlich-Frazier A, Pan J, Shabman RS, Chiu W, Davey RA, Otwinowski Z, Basler CF, Amarasinghe GK. An Intrinsically Disordered Peptide from Ebola Virus VP35 Controls Viral RNA Synthesis by Modulating Nucleoprotein-RNA Interactions. Cell Rep. 2015 Apr.11(3):376-389. Pubmedid: 25865894. Pmcid: PMC4599368.
Brown CS, Lee MS, Leung DW, Wang T, Xu W, Luthra P, Anantpadma M, Shabman RS, Melito LM, MacMillan KS, Borek DM, Otwinowski Z, Ramanan P, Stubbs AJ, Peterson DS, Binning JM, Tonelli M, Olson MA, Davey RA, Ready JM, Basler CF, Amarasinghe GK. In silico derived small molecules bind the filovirus VP35 protein and inhibit its polymerase cofactor activity. J Mol Biol. 2014 May.426(10):2045-2058. Pubmedid: 24495995. Pmcid: PMC4163021.
Binning JM, Wang T, Luthra P, Shabman RS, Borek DM, Liu G, Xu W, Leung DW, Basler CF, Amarasinghe GK. Development of RNA aptamers targeting Ebola virus VP35. Biochemistry-Us. 2013 Nov.52(47):8406-8419. Pubmedid: 24067086. Pmcid: PMC3909728.
Shabman RS, Hoenen T, Groseth A, Jabado O, Binning JM, Amarasinghe GK, Feldmann H, Basler CF. An upstream open reading frame modulates ebola virus polymerase translation and virus replication. PLoS Pathog. 2013 Jan.9(1):e1003147. Pubmedid: 23382680. Pmcid: PMC3561295.
Anderson MJ, Lonergan SM, Fedler CA, Prusa KJ, Binning JM, Huff-Lonergan E. Profile of biochemical traits influencing tenderness of muscles from the beef round. Meat Sci. 2012 Jul.91(3):247-254. Pubmedid: 22386323.
Binning JM, Leung DW, Amarasinghe GK. Aptamers in virology: recent advances and challenges. Front Microbiol. 2012 Feb.3:29. Pubmedid: 22347221. Pmcid: PMC3274758.
Prins KC, Binning JM, Shabman RS, Leung DW, Amarasinghe GK, Basler CF. Basic residues within the ebolavirus VP35 protein are required for its viral polymerase cofactor function. J Virol. 2010 Oct.84(20):10581-10591. Pubmedid: 20686031. Pmcid: PMC2950600.

Grants

Title: Developing HPV-targeting nanobodies
Sponsor: Moffitt Cancer Center
PI: Binning, J.
Title: Biophysical basis for enzyme mediated deglycation in protein repair
Sponsor: Nat Institutes of Health
PI: Binning, J.
Title: Development of a novel, non-BCMA targeting CAR-T cell therapy for the treatment of multiple myeloma
Sponsor: Moffitt Cancer Center
PI (Contact): Freeman, C., PI (MPI): Binning, J.
Title: The role of HPV E1 in regulating the NRF2-KEAP1 pathway
Sponsor: Nat Institutes of Health
PI: Binning, J.
Title: Biophysical basis for enzyme mediated deglycation in protein repair
Sponsor: Nat Institutes of Health
PI: Binning, J.

Jennifer Binning, PhD