Analytic Microscopy

Shared Resources

Purpose
The Analytic Microscopy Core’s (AMC) purpose is to provide access to the latest optical microscopy imaging technologies and expertise. The Core provides training and assisted support for many of its microscopes and imaging analysis software. Core staff members are available to assist investigators in experimental and scientific design of their imaging experiments so that the correct reagents, staining protocols and imaging techniques are employed to best address the scientific questions of the investigator. The AMC staff work closely with members to design, image, and analyze experiments, allowing members to obtain high-quality and reproducible data. Further, the AMC collaborates with other Shared Resource Facilities at MCC to manage complex projects and to ensure proper handoffs of materials and data.

Services
Leica SP5 AOBS Multiphoton Confocal Microscope:

  • 3D imaging and reconstruction
  • Colocalization assays
  • Multiphoton imaging
  • FRET & FRAP
  • Calcium imaging
  • Second Harmonic Generation

 Leica SP8 Confocal Microscope:

  • Colocalization assays
  • 3D imaging and reconstruction
  • HyD detector
  • An incubation/hypoxia chamber is available for live cell imaging

Widefield Fluorescence Microscopes:

  • Fully automated upright and inverted Zeiss microscopes
  • Mosaic and z-stack acquisition with post image processing
  • Fully enclosed incubation chamber for long term time lapse live cell imaging
  • Apotome available for pseudo-confocal imaging
  • EVOS FL and EVOS Auto FL systems for easy acquisition

Intravital Microscopy:

  • Live animal imaging via dorsal skin flap or cranial window chambers
  • Analyze tumor growth, invasion, collagen or vasculature modeling longitudinally
  • Real-Time analysis of angiogenesis, blood flow, vascular permeability, drug/agent kinetics
  • Real time microscopy quantification of pH or other metabolomic gradients

Aperio ScanScope XT Slide Scanner:

  • Holds up to 120 slides; scan time 10 slides/hour
  • Segments TMAs into individual cores
  • Spectrum software capable of pixel, nuclear and membrane algorithms
  • Genie software capable of segmenting regions of interest for IHC analysis

Incucyte Zoom Two Color HD:

  • High throughput live cell microscope with phase contrast, green/red FL
  • 96-well Wound Maker for wound healing assays
  • Various assay kits are available through Essen Bioscience

Image Analysis:

  • Definiens Developer
  • Definiens Tissue Studio
  • Inform
  • Imaris
  • AutoAligner
  • AutoDeblur
  • Metamorph
  • Image Pro Plus

Also available:

  • Laser Capture Microdissection
  • Comet Assay Imaging and Analysis
  • Stereoscopy

Location
The AMC is located in the Moffitt Research Center (MRC) on the 4th, Room 4058. An additional satellite facility is located in the Tabile Research Building (SRB) on the ground floor, Room 20307. This facility houses the Leica SP5 AOBS Multiphoton Confocal Microscope.

Scheduling
The AMC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for all Core requests, scheduling, billing, and usage tracking.

Publications
All publications based on work conducted in the Analytic Microscopy Core should acknowledge the facility.  A suggested statement is as follows:

This work has been supported in part by the Analytic Microscopy Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute.

If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.

Contacts
Scientific Director
Marilyn Bui, MD, PhD - marilyn.bui@moffitt.org

Core Facility Manager    
Joseph Johnson - joseph.johnson@moffitt.org
Office: 813-745-8710
Lab Number: 813-745-6157