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The Gene Targeting Core Facility was established in 2016 and supports the Moffitt Research Community with the production of genetically engineered mouse models. The facility is managed by Jose Gonzalez who has more than 20 years of experience in generating transgenic animals.
The goal of the facility is to offer a streamlined, efficient, and affordable array of state-of-the-art transgenic approaches to create cancer mouse models for in vivo biological and preclinical studies.
The facility is located in the vivarium in the Stabile Research Building, where it occupies two adjacent rooms for embryo manipulation and housing of animals. This assures the highest success of transgenic mouse production, eliminates the need for quarantine, and allows for easy transfer of transgenic mice to the investigators. In addition, the Gene Targeting Core Facility performs murine embryonic stem cell work, both derivation of new cell lines and gene targeting, in a dedicated cell culture room.
Capabilities and Services
- Assisting investigators with targeting strategies and vector design
- Derivation of embryonic stem cells
- Targeting of embryonic stem cells (traditional targeting vectors or CRISPR)
- Blastocyst injection
- 8-cell injection
- Tetraploid complementation
- Pronuclear injection (traditional transgenes or CRISPR)
- In vitro fertilization
- Mouse strain Rederivation
- Embryo and sperm cryopreservation
Gene Targeting Core Facility Staff
Scientific Director: Florian A. Karreth, PhD
Core Facility Manager: Jose G. Gonzalez, DVM
Research Associate III: Arianna Nenci, PhD
This work has been supported in part by the Flow Cytometry Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292)