Proteomics and Metabolomics

Shared Resources

The Proteomics and Metabolomics Core supports investigators with the experience of highly trained staff as well as instrumentation for proteomics studies, including protein and peptide separations, robotics for protein sampling and digestion, MS instrumentation for protein and peptide analysis, and data systems, software, and bioinformatics tools for data archival and analysis. The Proteomics and Metabolomics Core performs routine analytical proteomics services, i.e., protein identification, and also provides platforms for functional proteomics using a variety of strategies for protein separation, sub-proteome enrichment, post-translational modification analysis (PTM), and quantitation.

Separations / Enrichment:

SDS, HPLC (SCX, Basic pH Reverse Phase), Titanium Dioxide/IMAC, Capture for PTM Profiling, Activity-Based Protein Profiling (ABPP)

Sample Clean-up:

Peptide Ziptip (Reverse Phase, SCX), Peptide Sep-Pak, Size Exclusion, Ultrafiltration

Peptide Synthesis:

Standard Peptides, Heavy Isotope-Labeled Peptides, Peptides with Amino Acid Substitutions, and Post-Translationally Modified Peptides (e.g. phosphopeptides)

Mass Spectrometry:

MALDI-MS & MS/MS: Mass Analysis
LC-MS/MS: Protein ID, PTM
LC-MRM/LC-PRM: Relative & Absolute Quantification
Quantification: Label Free, Metabolic Labeling, Chemical Labeling

The PMC is located in the Moffitt Research Center (MRC) on the 1st floor, Room 1296.

The PMC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for billing and usage tracking.

All publications based on work conducted in the Proteomics and Metabolomics Core should acknowledge the facility.  A suggested statement is as follows:

If a Core staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.

Scientific Director
John Koomen, PhD -
Office: 813-745-8524

Core Facility Manager
Bin Fang, PhD -
Office: 813-745-2965

This work has been supported in part by the Proteomics and Metabolomics Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292)