Flow Cytometry

Shared Resources

Purpose
The Flow Cytometry provides state-of-the-art flow cytometry services and technologies to Moffitt Cancer Center members and their collaborators. Flow cytometry is an indispensable analytical tool for cell biology, immunology, and translational research efforts. Technologies provided by the core allow users to accurately analyze multiple characteristics of biological targets, as well as an ability to rapidly purify target populations to assess specific cellular functions and molecular characterization of isolated cell population.

Services

  • Multi-parameter analysis including DNA cell cycle, ploidy, apoptosis, viability, phenotyping, kinetics, cytokines, fluorescent proteins, proliferation, signal transduction, bead array, and morphology.
  • Cell sorting
  • Data analysis and graphics
  • Publication-quality documents
  • Instrument training and project consultation
  • Courses and workshops
  • Current literature base including cytometry journals, manufacturer information on instrumentation, methodology, protocols, techniques, and the latest vendor products and services.

Instruments

  • FACSCalibur (2)
  • Custom LSRII (2)
  • FACSCanto II
  • FACSAria SORP (2)
  • BioProtect IV Biosafety Cabinet (BSL2 level cell sorting)
  • ImageStreamX MarkII
  • iQue Screener Plus
  • MACSQuant YVB
  • Luminex 100
  • autoMACS Pro Separator

Hours of Operation
The Flow Cytometry Core is open 24 hours a day, seven days a week. Core Staff are available for assisted services Monday-Friday, 9:00am-6:00pm.  Assisted after-hours usage must be scheduled in advance and approved by the Core Facility Manager. After-hours, unassisted usage on the analyzers is available for trained and approved users. Usage is on a first-come, first-serve basis; however, priority is given to Moffitt Cancer Center members with peer-reviewed funded projects.

Location
The Flow Cytometry Core is located in the Moffitt Research Center (MRC) on the 1st floor, Room 1295. An additional satellite facility is located in the MRC on the fourth floor, Room 4024. This facility houses one FACSCalibur to be used by trained and approved users only.  Users must have an access badge to enter this room.

Scheduling
The FCC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for all Core requests, scheduling, billing, and usage tracking.

Publications
All publications based on work conducted in the Flow Cytometry Core Facility should acknowledge the facility.  A suggested statement is as follows:

This work has been supported in part by the Flow Cytometry Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute.

If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.

Contacts
Scientific Director
Julie Djeu, Ph.D. - julie.djeu@moffitt.org
Office: 813-745-3041

Core Facility Manager  
Jodi Kroeger - jodi.kroeger@moffitt.org     
Office: 813-745-6705
Lab: 813-745-2005