Purpose
The Chemical Biology Core (CBC) is comprised of two functional units: the Chemistry Division and the Structural Biology Division. The primary purpose of the Chemistry division is to assist, identify and optimize chemical probes and new lead compounds that may benefit both biochemical mechanistic studies and drug discovery & development. The primary purpose of the Structural Biology division is to assist with experiments related to protein purification, binding characterization, and structure determination. The two divisions work together to provide complementary approaches for identifying novel therapeutic targets and anti-cancer agents.
Equipment
- GE AktaPure FPCL for purifying proteins (2 instruments)
- Applied Biosystems QuantStudio 6 Flex RT-PCR
- Monolith NT.115 microscale thermophoresis
- GE Healthcare BiaCore T200 SPR
- MicroCal-iTC200*
- Envision multi-label multi-well plate readers
- Crystallization lab
- Rigaku X-ray generator
- TTP Labtech Mosquito Crystallization robot
- 500 MHz NMR with cryoprobe
- TOF-LCMS
- HPLC-MS (2 instruments)
Services
- Production of functional proteins in mg quantities for biochemical and biophysical studies; services include all steps from construct design to purification of homogenous protein.
- Crystallization of proteins, protein-small molecule complexes, protein-protein complexes, or protein-DNA complexes, and determination their 3-dimensional structures using X-ray crystallography.
- Perform quantitative binding studies of protein-ligand interactions using isothermal titration calorimetry (ITC), differential scanning fluorimetry (DSF), fluorescence polarization (FP), surface plasmon resonance (SPR), and/or microscale thermophoresis (MST).
- Synthesis: Re-synthesis of hits and leads to confirm structure and purity; medicinal chemistry and focused library design and synthesis for structure-activity relationships & lead optimization; synthesis of difficult-to-obtain probes/drugs for proof-of-principle studies; synthesis of biotinylated compounds/probes for chemical proteomics studies, multi-gram scale synthesis of potent compounds for pre-clinical in vivo studies.
- Synthesis of functional chemical probes for chemical proteomics studies for cancer target validation
- Pro-Drug synthesis and salt formation of potent compounds for preclinical studies.
- Analytical chemistry, solubility and stability assessments for lead compounds that are used for in vivo studies.
- Nuclear Magnetic Resonance (NMR), Mass Spectrometers (HPLC-MS and TOF-LCMS) instrumentation.
Location
The Chemical Biology Core, Chemistry Division, is located in the Moffitt Research Center (MRC) on the fourth floor, room 4017. The Structural Biology Division is located in the Stabile Research Building (SRB) on the fourth floor, room 24208.
Scheduling
The CBC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for billing and usage tracking.
Publications
All publications based on work conducted in the Chemical Biology Core should acknowledge the facility. A suggested statement is found below. If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.
This work has been supported in part by the Chemical Biology Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292)
Contacts
Scientific Directors
Harshani Lawrence, PhD
harshani.lawrence@moffitt.org
Office: 813-745-6076
Jennifer Binning, PhD
jennifer.binning@moffitt.org
Office: 813-745-3695
Staff Scientist
Timothy Tran, PhD
timothy.tran@moffitt.org
Office: 813-745-3580
