Charles Chalfant, PhD

Associations

• Cancer Biology & Evolution Program

• Ung J, Tan SF, Fox TE, Shaw JJ, Taori M, Horton BJ, Golla U, Sharma A, Szulc ZM, Wang HG, Chalfant CE, Cabot MC, Claxton DF, Loughran TP, Feith DJ. Acid Ceramidase Inhibitor LCL-805 Antagonizes Akt Signaling and Promotes Iron-Dependent Cell Death in Acute Myeloid Leukemia. bioRxiv. 2023 Oct. Pubmedid: 37961314. Pmcid: PMC10634704.
• Kong X, Patel NA, Chalfant CE, Cooper DR. Ceramide synthesis regulates biogenesis and packaging of exosomal MALAT1 from adipose derived stem cells, increases dermal fibroblast migration and mitochondrial function. Cell Commun Signal. 2023 Aug.21(1):221. Pubmedid: 37620957. Pmcid: PMC10463839.
• Vu NT, Kim M, Stephenson DJ, MacKnight HP, Chalfant CE. Ceramide Kinase Inhibition Drives Ferroptosis and Sensitivity to Cisplatin in Mutant KRAS Lung Cancer by Dysregulating VDAC-Mediated Mitochondria Function. Mol Cancer Res. 2022 Sep.20(9):1429-1442. Pubmedid: 35560154. Pmcid: PMC9444881.
• Madanayake TW, Welsh EA, Darville LNF, Koomen JM, Chalfant CE, Haura EB, Robinson TJ. Inhibition of Epidermal Growth Factor Receptor Signaling by Antisense Oligonucleotides as a Novel Approach to Epidermal Growth Factor Receptor Inhibition. Nucleic Acid Ther. 2022 Oct.32(5):391-400. Pubmedid: 35861718. Pmcid: PMC9595651.
• Mondal K, Porter H, Cole Ii J, Pandya HK, Basu SK, Khanam S, Chiu CY, Shah V, Stephenson DJ, Chalfant CE, Mandal N. Correction: Hydroxychloroquine Causes Early Inner Retinal Toxicity and Affects Autophagosome-Lysosomal Pathway and Sphingolipid Metabolism in the Retina. Mol Neurobiol. 2022 Oct.59(10):6611. Pubmedid: 35904716.
• Mondal K, Porter H, Cole J, Pandya HK, Basu SK, Khanam S, Chiu CY, Shah V, Stephenson DJ, Chalfant CE, Mandal N. Hydroxychloroquine Causes Early Inner Retinal Toxicity and Affects Autophagosome-Lysosomal Pathway and Sphingolipid Metabolism in the Retina. Mol Neurobiol. 2022 Jun.59(6):3873-3887. Pubmedid: 35426574.
• McGlone CL, Christian L, Schmeusser B, Liu L, Chalfant CE, Stephensen DJ, Sherwin CM, Rapp CM, Sattouf Z, Rohan CA, Morris C, Chen Y, Travers JB. Evidence for Systemic Reactive Oxygen Species in UVB-mediated Microvesicle Formation. Photochem Photobiol. 2022 Jan.98(1):242-247. Pubmedid: 34324712. Pmcid: PMC8799769.
• Kim M, Vu NT, Wang X, Bulut GB, Wang MH, Uram-Tuculescu C, Pillappa R, Kim S, Chalfant CE. Caspase 9b Drives Cellular Transformation, Lung Inflammation, and Lung Tumorigenesis. Mol Cancer Res. 2022 Aug.20(8):1284-1294. Pubmedid: 35412615. Pmcid: PMC9356980.
• Maus KD, Stephenson DJ, Ali AN, MacKnight HP, Huang HJ, Serrats J, Kim M, Diegelmann RF, Chalfant CE. Ceramide kinase regulates acute wound healing by suppressing 5-oxo-ETE biosynthesis and signaling via its receptor OXER1. J Lipid Res. 2022 Apr.63(4):100187. Pubmedid: 35219746. Pmcid: PMC8980959.
• Gowda B Gowda S, Gowda D, Kain V, Chiba H, Hui SP, Chalfant CE, Parcha V, Arora P, Halade G. Sphingosine-1-phosphate interactions in the spleen and heart reflect extent of cardiac repair in mice and failing human hearts. Am J Physiol Heart Circ Physiol. 2021 Sep.321(3):H599-H611. Pubmedid: 34415189. Pmcid: PMC8461844.
• Ali T, Lei X, Barbour SE, Koizumi A, Chalfant CE, Ramanadham S. Alterations in β-Cell Sphingolipid Profile Associated with ER Stress and iPLA2β: Another Contributor to β-Cell Apoptosis in Type 1 Diabetes. Molecules. 2021 Oct.26(21). Pubmedid: 34770770. Pmcid: PMC8587436.
• Mondal K, Takahashi H, Cole J, Del Mar NA, Li C, Stephenson DJ, Allegood J, Cowart LA, Chalfant CE, Reiner A, Mandal N. Systemic Elevation of n-3 Polyunsaturated Fatty Acids (n-3-PUFA) Is Associated with Protection against Visual, Motor, and Emotional Deficits in Mice following Closed-Head Mild Traumatic Brain Injury. Mol Neurobiol. 2021 Nov.58(11):5564-5580. Pubmedid: 34365584. Pmcid: PMC8655834.
• Vargas I, Stephenson DJ, Baldwin M, Gaut JP, Chalfant CE, Pan H, Wickline SA. Sustained local inhibition of thrombin preserves renal microarchitecture and function after onset of acute kidney injury. Nanomedicine. 2021 Nov.38:102449. Pubmedid: 34303838. Pmcid: PMC8541929.
• Hirano Y, Gao YG, Stephenson DJ, Vu NT, Malinina L, Simanshu DK, Chalfant CE, Patel DJ, Brown RE. Correction: Structural basis of phosphatidylcholine recognition by the C2-Domain of cytosolic phospholipase A2α. Elife. 2021 Nov.10. Pubmedid: 34843431. Pmcid: PMC8629424.
• Naimi WA, Gumpf JJ, Cockburn CL, Camus S, Chalfant CE, Li PL, Carlyon JA. Functional inhibition or genetic deletion of acid sphingomyelinase bacteriostatically inhibits Anaplasma phagocytophilum infection in vivo. Pathog Dis. 2021 Jan.79(1). Pubmedid: 33220685. Pmcid: PMC7787905.
• Hirano Y, Gao YG, K Simanshu D, J Stephenson D, T Vu N, Malinina L, E Chalfant C, J Patel D, E Brown R. Purification of Cytosolic Phospholipase A2α C2-domain after Expression in Soluble Form in Escherichia coli. Bio Protoc. 2021 Feb.11(3):e3906. Pubmedid: 33732793. Pmcid: PMC7952920.
• Amraoui F, Hassani Lahsinoui H, Spijkers LJA, Vogt L, Peters SLM, Wijesinghe DS, Warncke UO, Chalfant CE, Ris-Stalpers C, van den Born BH, Afink GB. Plasma ceramide is increased and associated with proteinuria in women with pre-eclampsia and HELLP syndrome. Pregnancy Hypertens. 2020 Jan.19:100-105. Pubmedid: 31927322.
• Nelson AJ, Stephenson DJ, Cardona CL, Lei X, Almutairi A, White TD, Tusing YG, Park MA, Barbour SE, Chalfant C, Ramanadham S. Macrophage polarization is linked to Ca2+-independent phospholipase A2β-derived lipids and cross-cell signaling in mice. J Lipid Res. 2020 Feb.61(2):143-158. Pubmedid: 31818877. Pmcid: PMC6997598.
• Walsh SW, Reep DT, Alam SMK, Washington SL, Al Dulaimi M, Lee SM, Springel EH, Strauss JF, Stephenson DJ, Chalfant CE. Placental Production of Eicosanoids and Sphingolipids in Women Who Developed Preeclampsia on Low-Dose Aspirin. Reprod Sci. 2020 Dec.27(12):2158-2169. Pubmedid: 32557282. Pmcid: PMC7606383.
• Nelson AJ, Stephenson DJ, Bone RN, Cardona CL, Park MA, Tusing YG, Lei X, Kokotos G, Graves CL, Mathews CE, Kramer J, Hessner MJ, Chalfant CE, Ramanadham S. Lipid mediators and biomarkers associated with type 1 diabetes development. JCI Insight. 2020 Aug.5(16). Pubmedid: 32814707. Pmcid: PMC7455134.
• Mishra SK, Gao YG, Zou X, Stephenson DJ, Malinina L, Hinchcliffe EH, Chalfant CE, Brown RE. Emerging roles for human glycolipid transfer protein superfamily members in the regulation of autophagy, inflammation, and cell death. Prog Lipid Res. 2020 Apr.78:101031. Pubmedid: 32339554. Pmcid: PMC8350976.
• DeLigio JT, Stevens SC, Nazario-Munoz GS, MacKnight HP, Doe KK, Chalfant CE, Park MA. Serine/Arginine-Rich Splicing Factor 3 Modulates the Alternative Splicing of Cytoplasmic Polyadenylation Element Binding Protein 2. Mol Cancer Res. 2019 Sep.17(9):1920-1930. Pubmedid: 31138601. Pmcid: PMC6726571.
• Berwick ML, Dudley BA, Maus K, Chalfant CE. The Role of Ceramide 1-Phosphate in Inflammation, Cellular Proliferation, and Wound Healing. Adv Exp Med Biol. 2019 Sep.1159:65-77. Pubmedid: 31502200.
• Hirano Y, Gao YG, Stephenson DJ, Vu NT, Malinina L, Simanshu DK, Chalfant CE, Patel DJ, Brown RE. Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase A2α. Elife. 2019 May.8. Pubmedid: 31050338. Pmcid: PMC6550875.
• Cockburn CL, Green RS, Damle SR, Martin RK, Ghahrai NN, Colonne PM, Fullerton MS, Conrad DH, Chalfant CE, Voth DE, Rucks EA, Gilk SD, Carlyon JA. Functional inhibition of acid sphingomyelinase disrupts infection by intracellular bacterial pathogens. Life Sci Alliance. 2019 Mar.2(2). Pubmedid: 30902833. Pmcid: PMC6431796.
• Caslin HL, Abebayehu D, Abdul Qayum A, Haque TT, Taruselli MT, Paez PA, Pondicherry N, Barnstein BO, Hoeferlin LA, Chalfant CE, Ryan JJ. Lactic Acid Inhibits Lipopolysaccharide-Induced Mast Cell Function by Limiting Glycolysis and ATP Availability. J Immunol. 2019 Jul.203(2):453-464. Pubmedid: 31160535. Pmcid: PMC6734564.
• Mishra SK, Stephenson DJ, Chalfant CE, Brown RE. Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells. Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Feb.1864(2):158-167. Pubmedid: 30472325. Pmcid: PMC6448591.
• MacKnight HP, Stephenson DJ, Hoeferlin LA, Benusa SD, DeLigio JT, Maus KD, Ali AN, Wayne JS, Park MA, Hinchcliffe EH, Brown RE, Ryan JJ, Diegelmann RF, Chalfant CE. The interaction of ceramide 1-phosphate with group IVA cytosolic phospholipase A2 coordinates acute wound healing and repair. Sci Signal. 2019 Dec.12(610). Pubmedid: 31796632. Pmcid: PMC8103357.
• Karandashova S, Kummarapurugu AB, Zheng S, Chalfant CE, Voynow JA. Neutrophil elastase increases airway ceramide levels via upregulation of serine palmitoyltransferase. Am J Physiol Lung Cell Mol Physiol. 2018 Jan.314(1):L206-L214. Pubmedid: 29025713. Pmcid: PMC5866429.
• Stephenson DJ, Hoeferlin LA, Chalfant CE. Lipidomics in translational research and the clinical significance of lipid-based biomarkers. Transl Res. 2017 Nov.189:13-29. Pubmedid: 28668521. Pmcid: PMC5659874.
• Contaifer D, Carl DE, Warncke UO, Martin EJ, Mohammed BM, Van Tassell B, Brophy DF, Chalfant CE, Wijesinghe DS. Unsupervised analysis of combined lipid and coagulation data reveals coagulopathy subtypes among dialysis patients. J Lipid Res. 2017 Mar.58(3):586-599. Pubmedid: 27993949. Pmcid: PMC5335589.
• Shapiro BA, Vu NT, Shultz MD, Shultz JC, Mietla JA, Gouda MM, Yacoub A, Dent P, Fisher PB, Park MA, Chalfant CE. Melanoma Differentiation-associated Gene 7/IL-24 Exerts Cytotoxic Effects by Altering the Alternative Splicing of Bcl-x Pre-mRNA via the SRC/PKCδ Signaling Axis. J Biol Chem. 2016 Oct.291(41):21669-21681. Pubmedid: 27519412. Pmcid: PMC5076836.
• Johnson RM, Vu NT, Griffin BP, Gentry AE, Archer KJ, Chalfant CE, Park MA. The Alternative Splicing of Cytoplasmic Polyadenylation Element Binding Protein 2 Drives Anoikis Resistance and the Metastasis of Triple Negative Breast Cancer. J Biol Chem. 2015 Oct.290(42):25717-25727. Pubmedid: 26304115. Pmcid: PMC4646214.
• Mohammed BM, Fisher BJ, Huynh QK, Wijesinghe DS, Chalfant CE, Brophy DF, Fowler AA, Natarajan R. Resolution of sterile inflammation: role for vitamin C. Mediators Inflamm. 2015 Jun.2014:173403. Pubmedid: 25294953. Pmcid: PMC4175383.
• Dhall S, Do D, Garcia M, Wijesinghe DS, Brandon A, Kim J, Sanchez A, Lyubovitsky J, Gallagher S, Nothnagel EA, Chalfant CE, Patel RP, Schiller N, Martins-Green M. A novel model of chronic wounds: importance of redox imbalance and biofilm-forming bacteria for establishment of chronicity. PLoS One. 2014 Oct.9(10):e109848. Pubmedid: 25313558. Pmcid: PMC4196950.
• van den Elsen LW, Spijkers LJ, van den Akker RF, van Winssen AM, Balvers M, Wijesinghe DS, Chalfant CE, Garssen J, Willemsen LE, Alewijnse AE, Peters SL. Dietary fish oil improves endothelial function and lowers blood pressure via suppression of sphingolipid-mediated contractions in spontaneously hypertensive rats. J Hypertens. 2014 May.32(5):1050-1058. Pubmedid: 24569415. Pmcid: PMC4312004.
• Wijesinghe DS, Brentnall M, Mietla JA, Hoeferlin LA, Diegelmann RF, Boise LH, Chalfant CE. Ceramide kinase is required for a normal eicosanoid response and the subsequent orderly migration of fibroblasts. J Lipid Res. 2014 Jul.55(7):1298-1309. Pubmedid: 24823941. Pmcid: PMC4076082.
• Wijesinghe DS, Chalfant CE. Systems-Level Lipid Analysis Methodologies for Qualitative and Quantitative Investigation of Lipid Signaling Events During Wound Healing. Adv Wound Care (New Rochelle). 2013 Nov.2(9):538-548. Pubmedid: 24527363. Pmcid: PMC3816989.
• West NW, Garcia-Vargas A, Chalfant CE, Park MA. OSU-03012 sensitizes breast cancers to lapatinib-induced cell killing: a role for Nck1 but not Nck2. BMC Cancer. 2013 May.13:256. Pubmedid: 23706161. Pmcid: PMC3674920.
• Shaak TL, Wijesinghe DS, Chalfant CE, Diegelmann RF, Ward KR, Loria RM. Structural Stereochemistry of Androstene Hormones Determines Interactions with Human Androgen, Estrogen, and Glucocorticoid Receptors. Int J Med Chem. 2013 Mar.2013:203606. Pubmedid: 24729874. Pmcid: PMC3981456.
• Vu NT, Park MA, Shultz JC, Goehe RW, Hoeferlin LA, Shultz MD, Smith SA, Lynch KW, Chalfant CE. hnRNP U enhances caspase-9 splicing and is modulated by AKT-dependent phosphorylation of hnRNP L. J Biol Chem. 2013 Mar.288(12):8575-8584. Pubmedid: 23396972. Pmcid: PMC3605676.
• Ward KE, Bhardwaj N, Vora M, Chalfant CE, Lu H, Stahelin RV. The molecular basis of ceramide-1-phosphate recognition by C2 domains. J Lipid Res. 2013 Mar.54(3):636-648. Pubmedid: 23277511. Pmcid: PMC3617939.
• Hoeferlin LA, Wijesinghe DS, Chalfant CE. The role of ceramide-1-phosphate in biological functions. Handb Exp Pharmacol. 2013 Jul.(215):153-166. Pubmedid: 23579454. Pmcid: PMC5598467.
• Mietla JA, Wijesinghe DS, Hoeferlin LA, Shultz MD, Natarajan R, Fowler AA, Chalfant CE. Characterization of eicosanoid synthesis in a genetic ablation model of ceramide kinase. J Lipid Res. 2013 Jul.54(7):1834-1847. Pubmedid: 23576683. Pmcid: PMC3679386.
• Gomez-Arroyo J, Mizuno S, Szczepanek K, Van Tassell B, Natarajan R, dos Remedios CG, Drake JI, Farkas L, Kraskauskas D, Wijesinghe DS, Chalfant CE, Bigbee J, Abbate A, Lesnefsky EJ, Bogaard HJ, Voelkel NF. Metabolic gene remodeling and mitochondrial dysfunction in failing right ventricular hypertrophy secondary to pulmonary arterial hypertension. Circ Heart Fail. 2013 Jan.6(1):136-144. Pubmedid: 23152488. Pmcid: PMC3790960.
• Khanna S, Rink C, Ghoorkhanian R, Gnyawali S, Heigel M, Wijesinghe DS, Chalfant CE, Chan YC, Banerjee J, Huang Y, Roy S, Sen CK. Loss of miR-29b following acute ischemic stroke contributes to neural cell death and infarct size. J Cereb Blood Flow Metab. 2013 Aug.33(8):1197-1206. Pubmedid: 23632968. Pmcid: PMC3734770.
• Wilson JD, Broaddus WC, Dorn HC, Fatouros PP, Chalfant CE, Shultz MD. Metallofullerene-nanoplatform-delivered interstitial brachytherapy improved survival in a murine model of glioblastoma multiforme. Bioconjug Chem. 2012 Sep.23(9):1873-1880. Pubmedid: 22881865. Pmcid: PMC3448337.
• Norton SK, Wijesinghe DS, Dellinger A, Sturgill J, Zhou Z, Barbour S, Chalfant C, Conrad DH, Kepley CL. Epoxyeicosatrienoic acids are involved in the C(70) fullerene derivative-induced control of allergic asthma. J Allergy Clin Immun. 2012 Sep.130(3):761-769.e2. Pubmedid: 22664166. Pmcid: PMC3955256.
• Shultz JC, Vu N, Shultz MD, Mba MU, Shapiro BA, Chalfant CE. The Proto-oncogene PKCι regulates the alternative splicing of Bcl-x pre-mRNA. Mol Cancer Res. 2012 May.10(5):660-669. Pubmedid: 22522453. Pmcid: PMC3356487.
• Shultz JC, Chalfant CE. The flip-flop HuR: part of the problem or the solution in fighting cancer?. J Clin Invest. 2012 Jan.122(1):16-19. Pubmedid: 22201677. Pmcid: PMC3248312.
• Spijkers LJ, Janssen BJ, Nelissen J, Meens MJ, Wijesinghe D, Chalfant CE, De Mey JG, Alewijnse AE, Peters SL. Antihypertensive treatment differentially affects vascular sphingolipid biology in spontaneously hypertensive rats. PLoS One. 2012 Aug.6(12):e29222. Pubmedid: 22195025. Pmcid: PMC3240660.
• Wijesinghe DS, Mayton EK, Mietla JA, Mukherjee A, Wu J, Fang X, Chalfant CE. Characterization of lysophosphatidic acid subspecies produced by autotaxin using a modified HPLC ESI-MS/MS method. Anal Methods. 2011 Nov.3(12):2822-2828. Pubmedid: 24648853. Pmcid: PMC3956132.
• Goldsmith M, Daka A, Lamour NF, Mashiach R, Glucksam Y, Meijler MM, Chalfant CE, Zor T. A ceramide analog inhibits cPLA(2) activity and consequent PGE(2) formation in LPS-stimulated macrophages. Immunol Lett. 2011 Mar.135(1-2):136-143. Pubmedid: 21040745. Pmcid: PMC3026855.
• Shultz JC, Goehe RW, Murudkar CS, Wijesinghe DS, Mayton EK, Massiello A, Hawkins AJ, Mukerjee P, Pinkerman RL, Park MA, Chalfant CE. SRSF1 regulates the alternative splicing of caspase 9 via a novel intronic splicing enhancer affecting the chemotherapeutic sensitivity of non-small cell lung cancer cells. Mol Cancer Res. 2011 Jul.9(7):889-900. Pubmedid: 21622622. Pmcid: PMC3140550.
• Spijkers LJ, van den Akker RF, Janssen BJ, Debets JJ, De Mey JG, Stroes ES, van den Born BJ, Wijesinghe DS, Chalfant CE, MacAleese L, Eijkel GB, Heeren RM, Alewijnse AE, Peters SL. Hypertension is associated with marked alterations in sphingolipid biology: a potential role for ceramide. PLoS One. 2011 Dec.6(7):e21817. Pubmedid: 21818267. Pmcid: PMC3139577.
• Lamour NF, Wijesinghe DS, Mietla JA, Ward KE, Stahelin RV, Chalfant CE. Ceramide kinase regulates the production of tumor necrosis factor α (TNFα) via inhibition of TNFα-converting enzyme. J Biol Chem. 2011 Dec.286(50):42808-42817. Pubmedid: 22009748. Pmcid: PMC3234830.
• Shultz JC, Chalfant CE. Caspase 9b: a new target for therapy in non-small-cell lung cancer. Expert Rev Anticancer Ther. 2011 Apr.11(4):499-502. Pubmedid: 21504315.
• Goehe RW, Shultz JC, Murudkar C, Usanovic S, Lamour NF, Massey DH, Zhang L, Camidge DR, Shay JW, Minna JD, Chalfant CE. hnRNP L regulates the tumorigenic capacity of lung cancer xenografts in mice via caspase-9 pre-mRNA processing. J Clin Invest. 2010 Nov.120(11):3923-3939. Pubmedid: 20972334. Pmcid: PMC2964989.
• Shultz JC, Goehe RW, Wijesinghe DS, Murudkar C, Hawkins AJ, Shay JW, Minna JD, Chalfant CE. Alternative splicing of caspase 9 is modulated by the phosphoinositide 3-kinase/Akt pathway via phosphorylation of SRp30a. Cancer Res. 2010 Nov.70(22):9185-9196. Pubmedid: 21045158. Pmcid: PMC3059118.
• Wijesinghe DS, Allegood JC, Gentile LB, Fox TE, Kester M, Chalfant CE. Use of high performance liquid chromatography-electrospray ionization-tandem mass spectrometry for the analysis of ceramide-1-phosphate levels. J Lipid Res. 2010 Mar.51(3):641-651. Pubmedid: 19654423. Pmcid: PMC2817594.
• Raucci FJ, Wijesinghe DS, Chalfant CE, Baumgarten CM. Exogenous and endogenous ceramides elicit volume-sensitive chloride current in ventricular myocytes. Cardiovasc Res. 2010 Apr.86(1):55-62. Pubmedid: 20008476. Pmcid: PMC2836262.
• Lamour NF, Subramanian P, Wijesinghe DS, Stahelin RV, Bonventre JV, Chalfant CE. Ceramide 1-phosphate is required for the translocation of group IVA cytosolic phospholipase A2 and prostaglandin synthesis. J Biol Chem. 2009 Sep.284(39):26897-26907. Pubmedid: 19632995. Pmcid: PMC2785377.
• Wijesinghe DS, Subramanian P, Lamour NF, Gentile LB, Granado MH, Bielawska A, Szulc Z, Gomez-Munoz A, Chalfant CE. Chain length specificity for activation of cPLA2alpha by C1P: use of the dodecane delivery system to determine lipid-specific effects. J Lipid Res. 2009 Oct.50(10):1986-1995. Pubmedid: 19075030. Pmcid: PMC2739767.
• Gubern A, Barceló-Torns M, Barneda D, López JM, Masgrau R, Picatoste F, Chalfant CE, Balsinde J, Balboa MA, Claro E. JNK and ceramide kinase govern the biogenesis of lipid droplets through activation of group IVA phospholipase A2. J Biol Chem. 2009 Nov.284(47):32359-32369. Pubmedid: 19778898. Pmcid: PMC2781651.
• Lamour NF, Chalfant CE. Ceramide kinase and the ceramide-1-phosphate/cPLA2alpha interaction as a therapeutic target. Curr Drug Targets. 2008 Aug.9(8):674-682. Pubmedid: 18691014.
• Paronetto MP, Achsel T, Massiello A, Chalfant CE, Sette C. The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol. 2007 Mar.176(7):929-939. Pubmedid: 17371836. Pmcid: PMC2064079.
• Lamour NF, Stahelin RV, Wijesinghe DS, Maceyka M, Wang E, Allegood JC, Merrill AH, Cho W, Chalfant CE. Ceramide kinase uses ceramide provided by ceramide transport protein: localization to organelles of eicosanoid synthesis. J Lipid Res. 2007 Jun.48(6):1293-1304. Pubmedid: 17392267.
• Stahelin RV, Subramanian P, Vora M, Cho W, Chalfant CE. Ceramide-1-phosphate binds group IVA cytosolic phospholipase a2 via a novel site in the C2 domain. J Biol Chem. 2007 Jul.282(28):20467-20474. Pubmedid: 17472963.
• Payne SG, Oskeritzian CA, Griffiths R, Subramanian P, Barbour SE, Chalfant CE, Milstien S, Spiegel S. The immunosuppressant drug FTY720 inhibits cytosolic phospholipase A2 independently of sphingosine-1-phosphate receptors. Blood. 2007 Feb.109(3):1077-1085. Pubmedid: 17008548. Pmcid: PMC1785128.
• Subramanian P, Vora M, Gentile LB, Stahelin RV, Chalfant CE. Anionic lipids activate group IVA cytosolic phospholipase A2 via distinct and separate mechanisms. J Lipid Res. 2007 Dec.48(12):2701-2708. Pubmedid: 17890681.
• Barnstein BO, Li G, Wang Z, Kennedy S, Chalfant C, Nakajima H, Bunting KD, Ryan JJ. Stat5 expression is required for IgE-mediated mast cell function. J Immunol. 2006 Sep.177(5):3421-3426. Pubmedid: 16920984.
• Massiello A, Chalfant CE. SRp30a (ASF/SF2) regulates the alternative splicing of caspase-9 pre-mRNA and is required for ceramide-responsiveness. J Lipid Res. 2006 May.47(5):892-897. Pubmedid: 16505493.
• Kim WS, Chalfant CE, Garner B. Fine tuning therapeutic targeting of the sphingolipid biosynthetic pathway to treat atherosclerosis. Curr Vasc Pharmacol. 2006 Apr.4(2):151-154. Pubmedid: 16611156.
• Subramanian P, Stahelin RV, Szulc Z, Bielawska A, Cho W, Chalfant CE. Ceramide 1-phosphate acts as a positive allosteric activator of group IVA cytosolic phospholipase A2 alpha and enhances the interaction of the enzyme with phosphatidylcholine. J Biol Chem. 2005 May.280(18):17601-17607. Pubmedid: 15743759.
• Wijesinghe DS, Massiello A, Subramanian P, Szulc Z, Bielawska A, Chalfant CE. Substrate specificity of human ceramide kinase. J Lipid Res. 2005 Dec.46(12):2706-2716. Pubmedid: 16170208.
• Chalfant CE, Szulc Z, Roddy P, Bielawska A, Hannun YA. The structural requirements for ceramide activation of serine-threonine protein phosphatases. J Lipid Res. 2004 Mar.45(3):496-506. Pubmedid: 14657198.
• Pettus BJ, Bielawska A, Subramanian P, Wijesinghe DS, Maceyka M, Leslie CC, Evans JH, Freiberg J, Roddy P, Hannun YA, Chalfant CE. Ceramide 1-phosphate is a direct activator of cytosolic phospholipase A2. J Biol Chem. 2004 Mar.279(12):11320-11326. Pubmedid: 14676210.
• Massiello A, Salas A, Pinkerman RL, Roddy P, Roesser JR, Chalfant CE. Identification of two RNA cis-elements that function to regulate the 5' splice site selection of Bcl-x pre-mRNA in response to ceramide. J Biol Chem. 2004 Apr.279(16):15799-15804. Pubmedid: 14734550.
• Pettus BJ, Bielawska A, Spiegel S, Roddy P, Hannun YA, Chalfant CE. Ceramide kinase mediates cytokine- and calcium ionophore-induced arachidonic acid release. J Biol Chem. 2003 Oct.278(40):38206-38213. Pubmedid: 12855693.
• Jenkins GM, Cowart LA, Signorelli P, Pettus BJ, Chalfant CE, Hannun YA. Acute activation of de novo sphingolipid biosynthesis upon heat shock causes an accumulation of ceramide and subsequent dephosphorylation of SR proteins. J Biol Chem. 2002 Nov.277(45):42572-42578. Pubmedid: 12200446.
• Pettus BJ, Chalfant CE, Hannun YA. Ceramide in apoptosis: an overview and current perspectives. Biochim Biophys Acta. 2002 Dec.1585(2-3):114-125. Pubmedid: 12531544.
• Chalfant CE, Rathman K, Pinkerman RL, Wood RE, Obeid LM, Ogretmen B, Hannun YA. De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1. J Biol Chem. 2002 Apr.277(15):12587-12595. Pubmedid: 11801602.