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Andrew S. Brohl, MD

Academic Rank: Assistant Member

Overview

Dr. Brohl's research focus is in translational genomics, applying next-generation sequencing and assay technology to advance biologic discovery, identify novel targets for therapy, and assist clinical management.


Discipline

    • Sarcoma
    • Chemical Biology and Molecular Medicine Program

Education & Training

Board Certification:

  • Medical Oncology

Fellowship:

  • University of Miami Miller School of Medicine, MD -
  • Georgetown University, Resident - Internal Medicine
  • National Cancer Institute, Fellow - Hematology and Oncology
Research

Dr. Brohl's research focus is in translational genomics, applying next-generation sequencing and assay technology to advance biologic discovery, identify novel targets for therapy, and assist clinical management. Recent projects include genomic landscape discovery in sarcoma subtypes, identification of drug targets from high-throughput “omics” datatypes, and development of genomics-based personalized cancer therapy program.

Publications

  • Hicks JK, Henderson-Jackson E, Duggan J, Joyce DM, Brohl AS. Identification of a novel MTAP-RAF1 fusion in a soft tissue sarcoma. Diagn Pathol. 2018 Oct;13(1):77. Pubmedid: 30314519. Pmcid: PMC6186031.
  • Jinesh GG, Flores ER, Brohl AS. Chromosome 19 miRNA cluster and CEBPB expression specifically mark and potentially drive triple negative breast cancers. PLoS One. 2018 Oct;13(10):e0206008. Pubmedid: 30335837. Pmcid: PMC6193703.
  • Eroglu Z, Chen YA, Gibney GT, Weber JS, Kudchadkar RR, Khushalani NI, Markowitz J, Brohl AS, Tetteh LF, Ramadan H, Arnone G, Li J, Zhao X, Sharma R, Darville LNF, Fang B, Smalley I, Messina JL, Koomen JM, Sondak VK, Smalley KSM. Combined BRAF and HSP90 Inhibition in Patients with Unresectable -Mutant Melanoma. Clin Cancer Res. 2018 Nov;24(22):5516-5524. Pubmedid: 29674508. Pmcid: PMC6195480.
  • Mullinax JE, Hall M, Prabhakaran S, Weber J, Khushalani N, Eroglu Z, Brohl AS, Markowitz J, Royster E, Richards A, Stark V, Zager JS, Kelley L, Cox C, Sondak VK, Mulé JJ, Pilon-Thomas S, Sarnaik AA. Combination of Ipilimumab and Adoptive Cell Therapy with Tumor-Infiltrating Lymphocytes for Patients with Metastatic Melanoma. Front Oncol. 2018 Mar;8:44. Pubmedid: 29552542. Pmcid: PMC5840208.
  • Saller J, Walko CM, Millis SZ, Henderson-Jackson E, Makanji R, Brohl AS. Response to Checkpoint Inhibitor Therapy in Advanced Classic Kaposi Sarcoma: A Case Report and Immunogenomic Study. J Natl Compr Canc Ne. 2018 Jul;16(7):797-800. Pubmedid: 30006421.
  • Pollack MH, Betof A, Dearden H, Rapazzo K, Valentine I, Brohl AS, Ancell KK, Long GV, Menzies AM, Eroglu Z, Johnson DB, Shoushtari AN. Safety of resuming anti-PD-1 in patients with immune-related adverse events (irAEs) during combined anti-CTLA-4 and anti-PD1 in metastatic melanoma. Ann Oncol. 2018 Jan;29(1):250-255. Pubmedid: 29045547. Pmcid: PMC5834131.
  • Borinstein SC, Steppan D, Hayashi M, Loeb DM, Isakoff MS, Binitie O, Brohl AS, Bridge JA, Stavas M, Shinohara ET, Meyer WH, Reed DR, Wagner LM. Consensus and controversies regarding the treatment of rhabdomyosarcoma. Pediatr Blood Cancer. 2018 Feb;65(2). Pubmedid: 28905489.
  • Castillo B, Gibbs J, Brohl AS, Seminario-Vidal L. Checkpoint inhibitor-associated cutaneous small vessel vasculitis. JAAD Case Rep. 2018 Aug;4(7):675-677. Pubmedid: 30112452. Pmcid: PMC6092526.
  • Kahen EJ, Brohl A, Yu D, Welch D, Cubitt CL, Lee JK, Chen Y, Yoder SJ, Teer JK, Zhang YO, Wallace MR, Reed DR. Neurofibromin level directs RAS pathway signaling and mediates sensitivity to targeted agents in malignant peripheral nerve sheath tumors. Oncotarget. 2018 Apr;9(32):22571-22585. Pubmedid: 29854299. Pmcid: PMC5978249.
  • Brohl AS, Kahen E, Yoder SJ, Teer JK, Reed DR. The genomic landscape of malignant peripheral nerve sheath tumors: diverse drivers of Ras pathway activation. Sci Rep. 2017 Nov;7(1):14992. Pubmedid: 29118384. Pmcid: PMC5678116.
  • Cancer Genome Atlas Research Network, Brohl AS. Comprehensive and Integrated Genomic Characterization of Adult Soft Tissue Sarcomas. Cell. 2017 Nov;171(4):950-965.e28. Pubmedid: 29100075. Pmcid: PMC5693358.
  • Reed DR, Hayashi M, Wagner L, Binitie O, Steppan DA, Brohl AS, Shinohara ET, Bridge JA, Loeb DM, Borinstein SC, Isakoff MS. Treatment pathway of bone sarcoma in children, adolescents, and young adults. Cancer. 2017 Jun;123(12):2206-2218. Pubmedid: 28323337. Pmcid: PMC5485018.
  • Brohl AS, Patidar R, Turner CE, Wen X, Song YK, Wei JS, Calzone KA, Khan J. Frequent inactivating germline mutations in DNA repair genes in patients with Ewing sarcoma. Genet Med. 2017 08;19(8):955-958. Pubmedid: 28125078. Pmcid: PMC5529247.
  • Hoggard TM, Henderson-Jackson E, Bui MM, Caracciolo J, Teer JK, Yoder S, Binitie O, Gonzalez RJ, Brohl AS, Reed DR. Myoepithelial carcinoma with RB1 mutation: remarkable chemosensitivity to carcinoma of unknown origin therapy. BMC Cancer. 2017 04;17(1):250. Pubmedid: 28390395. Pmcid: PMC5385017.
  • Ballinger ML, Goode DL, Ray-Coquard I, James PA, Mitchell G, Niedermayr E, Puri A, Schiffman JD, Dite GS, Cipponi A, Maki RG, Brohl AS, Myklebost O, Stratford EW, Lorenz S, Ahn SM, Ahn JH, Kim JE, Shanley S, Beshay V, Randall RL, Judson I, Seddon B, Campbell IG, Young MA, Sarin R, Blay JY, O'Donoghue SI, Thomas DM. Monogenic and polygenic determinants of sarcoma risk: an international genetic study. Lancet Oncol. 2016 Sep;17(9):1261-1271. Pubmedid: 27498913.
  • Kothari N, Teer JK, Abbott AM, Srikumar T, Zhang Y, Yoder SJ, Brohl AS, Kim RD, Reed DR, Shibata D. Increased incidence of FBXW7 and POLE proofreading domain mutations in young adult colorectal cancers. Cancer. 2016 09;122(18):2828-2835. Pubmedid: 27244218. Pmcid: PMC5014625.
  • Chang W, Brohl A, Patidar R, Sindiri S, Shern JF, Wei JS, Song YK, Yohe ME, Gryder BE, Zhang S, Calzone KA, Shivaprasad N, Wen X, Badgett T, Miettinen M, Hartman KR, League-Pascual JC, Trahair T, Widemann BC, Merchant MS, Kaplan RN, Lin JC, Khan J. MultiDimensional ClinOmics for Precision Therapy of Children and Adolescent Young Adults with Relapsed and Refractory Cancer: A Report from the Center for Cancer Research. Clin Cancer Res. 2016 08;22(15):3810-3820. Pubmedid: 26994145. Pmcid: PMC4970946.
  • Uzilov AV, Ding W, Fink MY, Antipin Y, Brohl AS, Davis C, Lau CY, Pandya C, Shah H, Kasai Y, Powell J, Micchelli M, Castellanos R, Zhang Z, Linderman M, Kinoshita Y, Zweig M, Raustad K, Cheung K, Castillo D, Wooten M, Bourzgui I, Newman LC, Deikus G, Mathew B, Zhu J, Glicksberg BS, Moe AS, Liao J, Edelmann L, Dudley JT, Maki RG, Kasarskis A, Holcombe RF, Mahajan M, Hao K, Reva B, Longtine J, Starcevic D, Sebra R, Donovan MJ, Li S, Schadt EE, Chen R. Development and clinical application of an integrative genomic approach to personalized cancer therapy. Genome Med. 2016 06;8(1):62. Pubmedid: 27245685. Pmcid: PMC4888213.
  • Brohl AS, Khushalani NI, Eroglu Z, Markowitz J, Thapa R, Chen YA, Kudchadkar R, Weber JS. A phase IB study of ipilimumab with peginterferon alfa-2b in patients with unresectable melanoma. J Immunother Cancer. 2016;4:85. Pubmedid: 28031816. Pmcid: PMC5170897.
  • Brock CO, Brohl AS, Običan SG. Incidence, pathophysiology, and clinical manifestations of antiphospholipid syndrome. Birth Defects Res C Embryo Today. 2015 Sep;105(3):201-208. Pubmedid: 26396056.
  • Brohl AS, Stinson JR, Su HC, Badgett T, Jennings CD, Sukumar G, Sindiri S, Wang W, Kardava L, Moir S, Dalgard CL, Moscow JA, Khan J, Snow AL. Germline CARD11 Mutation in a Patient with Severe Congenital B Cell Lymphocytosis. J Clin Immunol. 2015 Jan;35(1):32-46. Pubmedid: 25352053. Pmcid: PMC4466218.
  • Brohl AS, Li L, Andikyan V, Običan SG, Cioffi A, Hao K, Dudley JT, Ascher-Walsh C, Kasarskis A, Maki RG. Age-stratified risk of unexpected uterine sarcoma following surgery for presumed benign leiomyoma. Oncologist. 2015 Apr;20(4):433-439. Pubmedid: 25765878. Pmcid: PMC4391766.
  • Brohl AS, Demicco EG, Mourtzikos K, Maki RG. Response to sunitinib of a gastrointestinal stromal tumor with a rare exon 12 PDGFRA mutation. Clin Sarcoma Res. 2015;5:21. Pubmedid: 26396737. Pmcid: PMC4578851.
  • Brohl AS, Solomon DA, Chang W, Wang J, Song Y, Sindiri S, Patidar R, Hurd L, Chen L, Shern JF, Liao H, Wen X, Gerard J, Kim JS, Lopez Guerrero JA, Machado I, Wai DH, Picci P, Triche T, Horvai AE, Miettinen M, Wei JS, Catchpool D, Llombart-Bosch A, Waldman T, Khan J. The genomic landscape of the Ewing Sarcoma family of tumors reveals recurrent STAG2 mutation. PLoS Genet. 2014 Jul;10(7):e1004475. Pubmedid: 25010205. Pmcid: PMC4091782.
  • Shern JF, Chen L, Chmielecki J, Wei JS, Patidar R, Rosenberg M, Ambrogio L, Auclair D, Wang J, Song YK, Tolman C, Hurd L, Liao H, Zhang S, Bogen D, Brohl AS, Sindiri S, Catchpoole D, Badgett T, Getz G, Mora J, Anderson JR, Skapek SX, Barr FG, Meyerson M, Hawkins DS, Khan J. Comprehensive genomic analysis of rhabdomyosarcoma reveals a landscape of alterations affecting a common genetic axis in fusion-positive and fusion-negative tumors. Cancer Discov. 2014 Feb;4(2):216-231. Pubmedid: 24436047. Pmcid: PMC4462130.
  • Solomon DA, Brohl AS, Khan J, Miettinen M. Clinicopathologic features of a second patient with Ewing-like sarcoma harboring CIC-FOXO4 gene fusion. Am J Surg Pathol. 2014 Dec;38(12):1724-1725. Pubmedid: 25321332. Pmcid: PMC4229425.
  • Farid M, Ahn L, Brohl A, Cioffi A, Maki RG. Consumptive coagulopathy in angiosarcoma: a recurrent phenomenon?. Sarcoma. 2014 Apr;2014:617102. Pubmedid: 24693222. Pmcid: PMC3945465.
  • Brohl AS, Song JY, Lieberman R, Gause B. Synchronous indolent primary gastrointestinal lymphomas managed successfully with conservative measures. Am J Ther. 20(5):549-553. Pubmedid: 22020085. Pmcid: PMC3266981.
  • Snyder M, Gangadhara S, Brohl AS, Ludlow S, Nanjappa S. Serotonin Syndrome Complicating Treatment of Ifosfamide Neurotoxicity With Methylene Blue. Cancer Control. 24(5):1073274817729070. Pubmedid: 28975823. Pmcid: PMC5937239.