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Lawrence Stern, PhD

Overview


Associations

    • Immuno-Oncology

Education & Training

Publications

  • Ghosh D, Pham TD, Nanaware PP, Sengupta D, Adler LN, Li CG, He X, O'Mara ME, Kantor AB, Nguyen KD, Yang Y, Eisenlohr LC, Jensen PE, Herzenberg LA, Stern LJ, Boyd SD, Ghosn EEB, Mellins ED. Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells. Cell Rep. 2022 Jan.38(4):110200. Pubmedid: 35081339.
  • Parra M, Herrera D, Calvo-Calle JM, Stern LJ, Parra-López CA, Butcher E, Franco M, Angel J. Circulating human rotavirus specific CD4 T cells identified with a class II tetramer express the intestinal homing receptors α4β7 and CCR9. Virology. 2014 Mar.452-453:191-201. Pubmedid: 24606696. Pmcid: PMC3989888.
  • Stadinski BD, Trenh P, Duke B, Huseby PG, Li G, Stern LJ, Huseby ES. Effect of CDR3 sequences and distal V gene residues in regulating TCR-MHC contacts and ligand specificity. J Immunol. 2014 Jun.192(12):6071-6082. Pubmedid: 24813203. Pmcid: PMC4097884.
  • Yin L, Stern LJ. Measurement of Peptide Binding to MHC Class II Molecules by Fluorescence Polarization. Curr Protoc Immunol. 2014 Aug.106:5.10.1-5.10.12. Pubmedid: 25081912. Pmcid: PMC4151172.
  • Yin L, Stern LJ. A novel method to measure HLA-DM-susceptibility of peptides bound to MHC class II molecules based on peptide binding competition assay and differential IC(50) determination. J Immunol Methods. 2014 Apr.406:21-33. Pubmedid: 24583195. Pmcid: PMC4029850.
  • Yin L, Stern LJ. HLA-DM Focuses on Conformational Flexibility Around P1 Pocket to Catalyze Peptide Exchange. Front Immunol. 2013 Oct.4:336. Pubmedid: 24146666. Pmcid: PMC3797982.
  • Yin L, Calvo-Calle JM, Cruz J, Newman FK, Frey SE, Ennis FA, Stern LJ. CD4+ T cells provide intermolecular help to generate robust antibody responses in vaccinia virus-vaccinated humans. J Immunol. 2013 Jun.190(12):6023-6033. Pubmedid: 23667112. Pmcid: PMC3679297.
  • Guce AI, Mortimer SE, Yoon T, Painter CA, Jiang W, Mellins ED, Stern LJ. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nat Struct Mol Biol. 2013 Jan.20(1):90-98. Pubmedid: 23222639. Pmcid: PMC3537886.
  • Holland CJ, Rizkallah PJ, Vollers S, Calvo-Calle JM, Madura F, Fuller A, Sewell AK, Stern LJ, Godkin A, Cole DK. Minimal conformational plasticity enables TCR cross-reactivity to different MHC class II heterodimers. Sci Rep. 2013 Feb.2:629. Pubmedid: 22953050. Pmcid: PMC3432979.
  • Farfán-Arribas DJ, Stern LJ, Rock KL. Using intein catalysis to probe the origin of major histocompatibility complex class I-presented peptides. Proc Natl Acad Sci U S A. 2012 Oct.109(42):16998-17003. Pubmedid: 23027972. Pmcid: PMC3479494.
  • Yin L, Calvo-Calle JM, Dominguez-Amorocho O, Stern LJ. HLA-DM constrains epitope selection in the human CD4 T cell response to vaccinia virus by favoring the presentation of peptides with longer HLA-DM-mediated half-lives. J Immunol. 2012 Oct.189(8):3983-3994. Pubmedid: 22966084. Pmcid: PMC3466378.
  • Painter CA, Stern LJ. Conformational variation in structures of classical and non-classical MHCII proteins and functional implications. Immunol Rev. 2012 Nov.250(1):144-157. Pubmedid: 23046127. Pmcid: PMC3471379.
  • Nastke MD, Becerra A, Yin L, Dominguez-Amorocho O, Gibson L, Stern LJ, Calvo-Calle JM. Human CD4+ T cell response to human herpesvirus 6. J Virol. 2012 May.86(9):4776-4792. Pubmedid: 22357271. Pmcid: PMC3347333.
  • Huseby ES, Stadinski BD, Trenh P, Stern LJ. Response to Garcia et al. Immunity. 2012 Jun.36(6):889-890. Pubmedid: 22705102. Pmcid: PMC4547481.
  • Yoon T, Macmillan H, Mortimer SE, Jiang W, Rinderknecht CH, Stern LJ, Mellins ED. Mapping the HLA-DO/HLA-DM complex by FRET and mutagenesis. Proc Natl Acad Sci U S A. 2012 Jul.109(28):11276-11281. Pubmedid: 22733780. Pmcid: PMC3396517.
  • Rice JM, Stern LJ, Guignon EF, Lawrence DA, Lynes MA. Antigen-specific T cell phenotyping microarrays using grating coupled surface plasmon resonance imaging and surface plasmon coupled emission. Biosens Bioelectron. 2012 Jan.31(1):264-269. Pubmedid: 22104646. Pmcid: PMC3249003.
  • Stadinski BD, Trenh P, Smith RL, Bautista B, Huseby PG, Li G, Stern LJ, Huseby ES. A role for differential variable gene pairing in creating T cell receptors specific for unique major histocompatibility ligands. Immunity. 2011 Nov.35(5):694-704. Pubmedid: 22101158. Pmcid: PMC3253227.
  • Painter CA, Negroni MP, Kellersberger KA, Zavala-Ruiz Z, Evans JE, Stern LJ. Conformational lability in the class II MHC 310 helix and adjacent extended strand dictate HLA-DM susceptibility and peptide exchange. Proc Natl Acad Sci U S A. 2011 Nov.108(48):19329-19334. Pubmedid: 22084083. Pmcid: PMC3228433.
  • Clement CC, Cannizzo ES, Nastke MD, Sahu R, Olszewski W, Miller NE, Stern LJ, Santambrogio L. An expanded self-antigen peptidome is carried by the human lymph as compared to the plasma. PLoS One. 2010 Mar.5(3):e9863. Pubmedid: 20360855. Pmcid: PMC2845622.
  • Ge X, Gebe JA, Bollyky PL, James EA, Yang J, Stern LJ, Kwok WW. Peptide-MHC cellular microarray with innovative data analysis system for simultaneously detecting multiple CD4 T-cell responses. PLoS One. 2010 Jun.5(6):e11355. Pubmedid: 20634998. Pmcid: PMC2902358.
  • Kim WM, Sigalov AB, Stern LJ. Pseudo-merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different-length TCRzeta fragments. Acta Crystallogr D Biol Crystallogr. 2010 Feb.66(Pt 2):163-175. Pubmedid: 20124696. Pmcid: PMC2815668.
  • Campbell JD, Buckland KF, McMillan SJ, Kearley J, Oldfield WL, Stern LJ, Grönlund H, van Hage M, Reynolds CJ, Boyton RJ, Cobbold SP, Kay AB, Altmann DM, Lloyd CM, Larché M. Peptide immunotherapy in allergic asthma generates IL-10-dependent immunological tolerance associated with linked epitope suppression. J Exp Med. 2009 Jul.206(7):1535-1547. Pubmedid: 19528258. Pmcid: PMC2715096.
  • Stern LJ, Calvo-Calle JM. HLA-DR: molecular insights and vaccine design. Curr Pharm Des. 2009 Dec.15(28):3249-3261. Pubmedid: 19860674. Pmcid: PMC3615543.
  • Vollers SS, Stern LJ. Class II major histocompatibility complex tetramer staining: progress, problems, and prospects. Immunology. 2008 Mar.123(3):305-313. Pubmedid: 18251991. Pmcid: PMC2433336.
  • Painter CA, Cruz A, López GE, Stern LJ, Zavala-Ruiz Z. Model for the peptide-free conformation of class II MHC proteins. PLoS One. 2008 Jun.3(6):e2403. Pubmedid: 18545669. Pmcid: PMC2408972.
  • Strug I, Calvo-Calle JM, Green KM, Cruz J, Ennis FA, Evans JE, Stern LJ. Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor. J Proteome Res. 2008 Jul.7(7):2703-2711. Pubmedid: 18507432. Pmcid: PMC2515592.
  • Sigalov AB, Kim WM, Saline M, Stern LJ. The intrinsically disordered cytoplasmic domain of the T cell receptor zeta chain binds to the nef protein of simian immunodeficiency virus without a disorder-to-order transition. Biochemistry-Us. 2008 Dec.47(49):12942-12944. Pubmedid: 19012413. Pmcid: PMC3226742.
  • Stern LJ. Characterizing MHC-associated peptides by mass spectrometry. J Immunol. 2007 Sep.179(5):2667-2668. Pubmedid: 17709475.
  • Calvo-Calle JM, Strug I, Nastke MD, Baker SP, Stern LJ. Human CD4+ T cell epitopes from vaccinia virus induced by vaccination or infection. PLoS Pathog. 2007 Oct.3(10):1511-1529. Pubmedid: 17937498. Pmcid: PMC2014795.