Proteomics

Shared Resources

Purpose
The Proteomics Core supports investigators with the experience of highly trained staff as well as instrumentation for proteomics studies, including protein and peptide separations, robotics for protein sampling and digestion, MS instrumentation for protein and peptide analysis, and data systems, software, and bioinformatics tools for data archival and analysis. The Proteomics Core performs routine analytical proteomics services, i.e., protein identification, and also provides platforms for functional proteomics using a variety of strategies for protein separation, sub-proteome enrichment, post-translational modification analysis (PTM), and quantitation.

Services
Separations / Enrichment:

SDS, HPLC (SCX, Basic pH Reverse Phase), Titanium Dioxide/IMAC, Capture for PTM Profiling, Activity-Based Protein Profiling (ABPP)

Sample Clean-up:

Peptide Ziptip (Reverse Phase, SCX), Peptide Sep-Pak, Size Exclusion, Ultrafiltration

Peptide Synthesis:

Standard Peptides, Heavy Isotope-Labeled Peptides, Peptides with Amino Acid Substitutions, and Post-Translationally Modified Peptides (e.g. phosphopeptides)

Mass Spectrometry:

MALDI-MS & MS/MS: Mass Analysis
LC-MS/MS: Protein ID, PTM
LC-MRM/LC-PRM: Relative & Absolute Quantification
Quantification: Label Free, Metabolic Labeling, Chemical Labeling

Location
The PC is located in the Moffitt Research Center (MRC) on the 1st floor, Room 1296.

Scheduling
The PC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for billing and usage tracking.

Publications
All publications based on work conducted in the Proteomics Core should acknowledge the facility.  A suggested statement is as follows:

This work has been supported in part by the Proteomics Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute.

If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.

Contacts
Scientific Director
John Koomen, PhD - john.koomen@moffitt.org
Office: 813-745-8524

Core Facility Manager
Bin Fang, PhD - bin.fang@moffitt.org
Office: 813-745-2965