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Antonio  Amelio

Antonio Amelio, PhD

Program: Tumor Microenvironment and Metastasis

Research Program: Cancer Biology & Evolution Program

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  • Overview

    Associations

    • Tumor Microenvironment and Metastasis
    • Head and Neck-Endocrine Oncology
    • Cancer Biology & Evolution Program
  • Research Interest

    We use an interdisciplinary approach that integrates a combination of bioinformatics, genetically engineered cell line and animal models, pharmacologic and molecular genetic analyses, and synthetic biology approaches to investigate tumor development and progression and identify therapeutic vulnerabilities.

  • Publications

    • Dickey BL, Putney RM, Schell MJ, Berglund AE, Amelio AL, Caudell JJ, Chung CH, Giuliano AR. IDENTIFICATION OF A BIOMARKER PANEL FROM GENOME-WIDE METHYLATION TO DETECT EARLY HPV-ASSOCIATED OROPHARYNGEAL CANCER. Cancer Prev Res (Phila). 2024 Jan. Pubmedid: 38286404.
    • Tasoulas J, Farquhar DR, Sheth S, Hackman T, Yarbrough WG, Agala CB, Koric A, Giraldi L, Fabianova E, Lissowska J, Świątkowska B, Vilensky M, Wünsch-Filho V, de Carvalho MB, López RVM, Holcátová I, Serraino D, Polesel J, Canova C, Richiardi L, Zevallos JP, Ness A, Pring M, Thomas SJ, Dudding T, Lee YA, Hashibe M, Boffetta P, Olshan AF, Divaris K, Amelio AL. Poor oral health influences head and neck cancer patient survival: an International Head and Neck Cancer Epidemiology Consortium pooled analysis. J Natl Cancer Inst. 2024 Jan.116(1):105-114. Pubmedid: 37725515. Pmcid: PMC10777670.
    • Marzban S, Srivastava S, Kartika S, Bravo R, Safriel R, Zarski A, Anderson A, Chung CH, Amelio AL, West J. Spatial interactions modulate tumor growth and immune infiltration. bioRxiv. 2024 Feb. Pubmedid: 38370722. Pmcid: PMC10871273.
    • Tasoulas J, Srivastava S, Xu X, Tarasova V, Maniakas A, Karreth FA, Amelio AL. Genetically engineered mouse models of head and neck cancers. Oncogene. 2023 Aug.42(35):2593-2609. Pubmedid: 37474617. Pmcid: PMC10457205.
    • Murphy RM, Tasoulas J, Porrello A, Carper MB, Tsai YH, Coffey AR, Kumar S, Zeng PY, Schrank TP, Midkiff BR, Cohen S, Salazar AH, Hayward MC, Hayes DN, Olshan A, Gupta GP, Nichols AC, Yarbrough WG, Pecot CV, Amelio AL. Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer. Cancer Res Commun. 2022 Sep.2(9):987-1004. Pubmedid: 36148399. Pmcid: PMC9491693.
    • Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA. Multiplexed bioluminescence microscopy via phasor analysis. Nat Methods. 2022 Jul.19(7):893-898. Pubmedid: 35739310.
    • Carper MB, Goel S, Zhang AM, Damrauer JS, Cohen S, Zimmerman MP, Gentile GM, Parag-Sharma K, Murphy RM, Sato K, Nickel KP, Kimple RJ, Yarbrough WG, Amelio AL. Activation of the CREB Coactivator CRTC2 by Aberrant Mitogen Signaling promotes oncogenic functions in HPV16 positive head and neck cancer. Neoplasia. 2022 Jul.29:100799. Pubmedid: 35504112. Pmcid: PMC9065880.
    • Zeng PYF, Cecchini MJ, Barrett JW, Shammas-Toma M, De Cecco L, Serafini MS, Cavalieri S, Licitra L, Hoebers F, Brakenhoff RH, Leemans CR, Scheckenbach K, Poli T, Wang X, Liu X, Laxague F, Prisman E, Poh C, Bose P, Dort JC, Shaikh MH, Ryan SEB, Dawson A, Khan MI, Howlett CJ, Stecho W, Plantinga P, Daniela da Silva S, Hier M, Khan H, MacNeil D, Mendez A, Yoo J, Fung K, Lang P, Winquist E, Palma DA, Ziai H, Amelio AL, Li SS, Boutros PC, Mymryk JS, Nichols AC. Immune-based classification of HPV-associated oropharyngeal cancer with implications for biomarker-driven treatment de-intensification. EBioMedicine. 2022 Dec.86:104373. Pubmedid: 36442320. Pmcid: PMC9706534.
    • Terajima M, Taga Y, Nakamura T, Guo HF, Kayashima Y, Maeda-Smithies N, Parag-Sharma K, Kim JS, Amelio AL, Mizuno K, Kurie JM, Yamauchi M. Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes. Sci Rep. 2022 Aug.12(1):14256. Pubmedid: 35995931. Pmcid: PMC9395344.
    • Sato K, Parag-Sharma K, Terajima M, Musicant AM, Murphy RM, Ramsey MR, Hibi H, Yamauchi M, Amelio AL. Lysyl hydroxylase 2-induced collagen cross-link switching promotes metastasis in head and neck squamous cell carcinomas. Neoplasia. 2021 Jun.23(6):594-606. Pubmedid: 34107376. Pmcid: PMC8192727.
    • Wang L, Lee DJ, Han H, Zhao L, Tsukamoto H, Kim YI, Musicant AM, Parag-Sharma K, Hu X, Tseng HC, Chi JT, Wang Z, Amelio AL, Ko CC. Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering. J Tissue Eng. 2021 Feb.12. Pubmedid: 33643604. Pmcid: PMC7894599.
    • Tasoulas J, Divaris K, Theocharis S, Farquhar D, Shen C, Hackman T, Amelio AL. Impact of Tumor Site and Adjuvant Radiotherapy on Survival of Patients with Adenoid Cystic Carcinoma: A SEER Database Analysis. Cancers (Basel). 2021 Feb.13(4). Pubmedid: 33546179. Pmcid: PMC7913220.
    • Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL. CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibition. Cell Rep. 2021 Feb.34(8):108768. Pubmedid: 33626346. Pmcid: PMC7955229.
    • Parag-Sharma K, Tasoulas J, Musicant AM, do Nascimento-Filho CHV, Zhu Z, Twomey C, Liu P, Castilho RM, Amelio AL. Synergistic efficacy of combined EGFR and HDAC inhibitors overcomes tolerance to EGFR monotherapy in salivary mucoepidermoid carcinoma. Oral Oncol. 2021 Apr.115:105166. Pubmedid: 33581505. Pmcid: PMC8026571.
    • Dorronsoro A, Santiago FE, Grassi D, Zhang T, Lai RC, McGowan SJ, Angelini L, Lavasani M, Corbo L, Lu A, Brooks RW, Garcia-Contreras M, Stolz DB, Amelio A, Boregowda SV, Fallahi M, Reich A, Ricordi C, Phinney DG, Huard J, Lim SK, Niedernhofer LJ, Robbins PD. Mesenchymal stem cell-derived extracellular vesicles reduce senescence and extend health span in mouse models of aging. Aging Cell. 2021 04.20(4):e13337. Pubmedid: 33728821. Pmcid: PMC8045949.
    • Parag-Sharma K, O'Banion CP, Henry EC, Musicant AM, Cleveland JL, Lawrence DS, Amelio AL. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol. 2020 Jan.9(1):1-9. Pubmedid: 31834783. Pmcid: PMC7875091.
    • Carper MB, Troutman S, Wagner BL, Byrd KM, Selitsky SR, Parag-Sharma K, Henry EC, Li W, Parker JS, Montgomery SA, Cleveland JL, Williams SE, Kissil JL, Hayes DN, Amelio AL. An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma. Cell Rep. 2019 Nov.29(6):1660-1674.e7. Pubmedid: 31693903. Pmcid: PMC6870917.
    • Tang Y, Parag-Sharma K, Amelio AL, Cao Y. A Bioluminescence Resonance Energy Transfer-Based Approach for Determining Antibody-Receptor Occupancy In Vivo. iScience. 2019 May.15:439-451. Pubmedid: 31121469. Pmcid: PMC6529791.
    • Saito T, Uzawa K, Terajima M, Shiiba M, Amelio AL, Tanzawa H, Yamauchi M. Aberrant Collagen Cross-linking in Human Oral Squamous Cell Carcinoma. J Dent Res. 2019 May.98(5):517-525. Pubmedid: 30786814.
    • Getz GI, Parag-Sharma K, Reside J, Padilla RJ, Amelio AL. Identification of NSDHL mutations associated with CHILD syndrome in oral verruciform xanthoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2019 Jul.128(1):60-69. Pubmedid: 31078502.
    • Tasoulas J, Rodon L, Kaye FJ, Montminy M, Amelio AL. Adaptive Transcriptional Responses by CRTC Coactivators in Cancer. Trends Cancer. 2019 Feb.5(2):111-127. Pubmedid: 30755304. Pmcid: PMC9682902.
    • Uzawa K, Amelio AL, Kasamatsu A, Saito T, Kita A, Fukamachi M, Sawai Y, Toeda Y, Eizuka K, Hayashi F, Kato-Kase I, Sunohara M, Iyoda M, Koike K, Nakashima D, Ogawara K, Endo-Sakamoto Y, Shiiba M, Takiguchi Y, Yamauchi M, Tanzawa H. Resveratrol Targets Urokinase-Type Plasminogen Activator Receptor Expression to Overcome Cetuximab-Resistance in Oral Squamous Cell Carcinoma. Sci Rep. 2019 Aug.9(1):12179. Pubmedid: 31434965. Pmcid: PMC6704133.
    • Fishbein L, Leshchiner I, Walter V, Danilova L, Robertson AG, Johnson AR, Lichtenberg TM, Murray BA, Ghayee HK, Else T, Ling S, Jefferys SR, de Cubas AA, Wenz B, Korpershoek E, Amelio AL, Makowski L, Rathmell WK, Gimenez-Roqueplo AP, Giordano TJ, Asa SL, Tischler AS, Pacak K, Nathanson KL, Wilkerson MD. Comprehensive Molecular Characterization of Pheochromocytoma and Paraganglioma. Cancer Cell. 2017 Feb.31(2):181-193. Pubmedid: 28162975. Pmcid: PMC5643159.
    • Chen Z, Li JL, Lin S, Cao C, Gimbrone NT, Yang R, Fu DA, Carper MB, Haura EB, Schabath MB, Lu J, Amelio AL, Cress WD, Kaye FJ, Wu L. cAMP/CREB-regulated LINC00473 marks LKB1-inactivated lung cancer and mediates tumor growth. J Clin Invest. 2016 Jun.126(6):2267-2279. Pubmedid: 27140397. Pmcid: PMC4887185.
    • Peña CG, Nakada Y, Saatcioglu HD, Aloisio GM, Cuevas I, Zhang S, Miller DS, Lea JS, Wong KK, DeBerardinis RJ, Amelio AL, Brekken RA, Castrillon DH. LKB1 loss promotes endometrial cancer progression via CCL2-dependent macrophage recruitment. J Clin Invest. 2015 Nov.125(11):4063-4076. Pubmedid: 26413869. Pmcid: PMC4639978.
    • Flaveny CA, Griffett K, El-Gendy Bel-D, Kazantzis M, Sengupta M, Amelio AL, Chatterjee A, Walker J, Solt LA, Kamenecka TM, Burris TP. Broad Anti-tumor Activity of a Small Molecule that Selectively Targets the Warburg Effect and Lipogenesis. Cancer Cell. 2015 Jul.28(1):42-56. Pubmedid: 26120082. Pmcid: PMC4965273.
    • Cao C, Gao R, Zhang M, Amelio AL, Fallahi M, Chen Z, Gu Y, Hu C, Welsh EA, Engel BE, Haura EB, Cress WD, Wu L, Zajac-Kaye M, Kaye FJ. Role of LKB1-CRTC1 on glycosylated COX-2 and response to COX-2 inhibition in lung cancer. J Natl Cancer Inst. 2015 Jan.107(1):358. Pubmedid: 25465874. Pmcid: PMC4271078.
    • Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, Cleveland JL, Amelio AL. Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis. Cancer Res. 2015 Dec.75(23):5023-5033. Pubmedid: 26424696. Pmcid: PMC4668208.
    • Bruno NE, Kelly KA, Hawkins R, Bramah-Lawani M, Amelio AL, Nwachukwu JC, Nettles KW, Conkright MD. Creb coactivators direct anabolic responses and enhance performance of skeletal muscle. Embo J. 2014 May.33(9):1027-1043. Pubmedid: 24674967. Pmcid: PMC4193935.
    • Doherty JR, Yang C, Scott KE, Cameron MD, Fallahi M, Li W, Hall MA, Amelio AL, Mishra JK, Li F, Tortosa M, Genau HM, Rounbehler RJ, Lu Y, Dang CV, Kumar KG, Butler AA, Bannister TD, Hooper AT, Unsal-Kacmaz K, Roush WR, Cleveland JL. Blocking lactate export by inhibiting the Myc target MCT1 Disables glycolysis and glutathione synthesis. Cancer Res. 2014 Feb.74(3):908-920. Pubmedid: 24285728. Pmcid: PMC3946415.
    • Fallahi M, Amelio AL, Cleveland JL, Rounbehler RJ. CREB targets define the gene expression signature of malignancies having reduced levels of the tumor suppressor tristetraprolin. PLoS One. 2014 Dec.9(12):e115517. Pubmedid: 25541715. Pmcid: PMC4277357.
    • Amelio AL, Fallahi M, Schaub FX, Zhang M, Lawani MB, Alperstein AS, Southern MR, Young BM, Wu L, Zajac-Kaye M, Kaye FJ, Cleveland JL, Conkright MD. CRTC1/MAML2 gain-of-function interactions with MYC create a gene signature predictive of cancers with CREB-MYC involvement. Proc Natl Acad Sci U S A. 2014 Aug.111(32):E3260-E3268. Pubmedid: 25071166. Pmcid: PMC4136562.
    • Jablonski JA, Amelio AL, Giacca M, Caputi M. The transcriptional transactivator Tat selectively regulates viral splicing. Nucleic Acids Res. 2010 Mar.38(4):1249-1260. Pubmedid: 19966273. Pmcid: PMC2831323.
    • Hollander JA, Im HI, Amelio AL, Kocerha J, Bali P, Lu Q, Willoughby D, Wahlestedt C, Conkright MD, Kenny PJ. Striatal microRNA controls cocaine intake through CREB signalling. Nature. 2010 Jul.466(7303):197-202. Pubmedid: 20613834. Pmcid: PMC2916751.
    • Amelio AL, Caputi M, Conkright MD. Bipartite functions of the CREB co-activators selectively direct alternative splicing or transcriptional activation. Embo J. 2009 Sep.28(18):2733-2747. Pubmedid: 19644446. Pmcid: PMC2750025.
    • Amelio AL, Miraglia LJ, Conkright JJ, Mercer BA, Batalov S, Cavett V, Orth AP, Busby J, Hogenesch JB, Conkright MD. A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling pathway. Proc Natl Acad Sci U S A. 2007 Dec.104(51):20314-20319. Pubmedid: 18077367. Pmcid: PMC2154428.
    • Amelio AL, McAnany PK, Bloom DC. A chromatin insulator-like element in the herpes simplex virus type 1 latency-associated transcript region binds CCCTC-binding factor and displays enhancer-blocking and silencing activities. J Virol. 2006 Mar.80(5):2358-2368. Pubmedid: 16474142. Pmcid: PMC1395413.
    • Amelio AL, Giordani NV, Kubat NJ, O'neil JE, Bloom DC. Deacetylation of the herpes simplex virus type 1 latency-associated transcript (LAT) enhancer and a decrease in LAT abundance precede an increase in ICP0 transcriptional permissiveness at early times postexplant. J Virol. 2006 Feb.80(4):2063-2068. Pubmedid: 16439563. Pmcid: PMC1367155.
    • Kubat NJ, Amelio AL, Giordani NV, Bloom DC. The herpes simplex virus type 1 latency-associated transcript (LAT) enhancer/rcr is hyperacetylated during latency independently of LAT transcription. J Virol. 2004 Nov.78(22):12508-12518. Pubmedid: 15507638. Pmcid: PMC525101.
  • Grants

    • Title: Biomarker Approach to Screening for the early detection of HPV-related Oropharyngeal Cancer (BASH OPC)
      Sponsor: Nat Institutes of Health
      PI (Contact): Giuliano, A., PI (MPI): Amelio, A.
    • Title: Molecular Characterization of the Salivary Adenoid Cystic Carcinoma Tumor Immune Landscape by Anatomic Subsite
      Sponsor: Adenoid Cystic Carcinoma Rsch Found (ACCRF)
      PI: Amelio, A.
    • Title: Role of CRTC1-MAML2 in Salivary Mucoepidermoid Carcinoma Pathobiology
      Sponsor: Nat Institutes of Health
      PI: Amelio, A.

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