Mildred E. Acevedo-Duncan, PhD

Where You Are:
Mildred E. Acevedo-Duncan, PhD

Office  (813) 972-2000

Education And Training
  • Postdoctoral Fellow, University of South Florida, Tampa, FL, 1986
  • Postdoctoral Fellow, University of Florida, Gainesville, FL, 1985
  • PhD, University of California, Riverside, 1983
  • MA, University of California, Riverside, 1979
  • BA, University of California, San Diego, 1977

My research investigates how protein kinase C (PKC)  isozymes interact to affect the cell cycle during cancer growth and development.  Information on the role of PKC isozymes in cell cycle regulation is important since PKC overproduction has been linked with the rapid growth rate of cancer cells. Our studies use human cancer cells in-vitro to determine how PKC inhibitors alone or in combination with other chemotherapeutics affect PKC,  cell proliferation and survival. The goals are to  identify PKC isozymes or PKC substrates which regulate the cell cycle in order to target and interfere with their activity, thereby providing selective therapeutic agents for controlling cancer cell proliferation.  Our research methods employ physical and biochemical isolation procedures, glioma PKC overexpressors, flow cytometry, scanning and transmission electron microscopy, and Western blotting.

  • Sajan MP, Acevedo-Duncan ME, Standaert ML, Ivey RA, Lee M, Farese RV. Akt-dependent phosphorylation of hepatic FoxO1 is compartmentalized on a WD40/ProF scaffold and is selectively inhibited by aPKC in early phases of diet-induced obesity. Diabetes. 2014 Aug;63(8):2690-2701. Pubmedid: 24705403. Pmcid: PMC4113067.
  • Sajan MP, Nimal S, Mastorides S, Acevedo-Duncan M, Kahn CR, Fields AP, Braun U, Leitges M, Farese RV. Correction of metabolic abnormalities in a rodent model of obesity, metabolic syndrome, and type 2 diabetes mellitus by inhibitors of hepatic protein kinase C-ι. Metabolism. 2012 Apr;61(4):459-469. Pubmedid: 22225955. Pmcid: PMC3786325.
  • Desai SR, Pillai PP, Patel RS, McCray AN, Win-Piazza HY, Acevedo-Duncan ME. Regulation of Cdk7 activity through a phosphatidylinositol (3)-kinase/PKC-ι-mediated signaling cascade in glioblastoma. Carcinogenesis. 2012 Jan;33(1):10-19. Pubmedid: 22021906.
  • Pillai P, Desai S, Patel R, Sajan M, Farese R, Ostrov D, Acevedo-Duncan M. A novel PKC-ι inhibitor abrogates cell proliferation and induces apoptosis in neuroblastoma. Int J Biochem Cell Biol. 2011 May;43(5):784-794. Pubmedid: 21315177.
  • Win HY, Acevedo-Duncan M. Role of protein kinase C-iota in transformed non-malignant RWPE-1 cells and androgen-independent prostate carcinoma DU-145 cells. Cell Prolif. 2009 Apr;42(2):182-194. Pubmedid: 19243387.
  • Win HY, Acevedo-Duncan M. Atypical protein kinase C phosphorylates IKKalphabeta in transformed non-malignant and malignant prostate cell survival. Cancer Lett. 2008 Nov;270(2):302-311. Pubmedid: 18571841.
  • Matthews JA, Belof JL, Acevedo-Duncan M, Potter RL. Glucosamine-induced increase in Akt phosphorylation corresponds to increased endoplasmic reticulum stress in astroglial cells. Mol Cell Biochem. 2007 Apr;298(1-2):109-123. Pubmedid: 17136481.
  • Matthews JA, Acevedo-Duncan M, Potter RL. Selective decrease of membrane-associated PKC-alpha and PKC-epsilon in response to elevated intracellular O-GlcNAc levels in transformed human glial cells. Biochim Biophys Acta. 2005 Apr;1743(3):305-315. Pubmedid: 15843043.
  • Bicaku E, Patel R, Acevedo-Duncan M. Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-iota in human glioma cells. Tissue Cell. 2005 Feb;37(1):53-58. Pubmedid: 15695176.
  • Acevedo-Duncan M, Russell C, Patel S, Patel R. Aloe-emodin modulates PKC isozymes, inhibits proliferation, and induces apoptosis in U-373MG glioma cells. Int Immunopharmacol. 2004 Dec;4(14):1775-1784. Pubmedid: 15531293.
  • Acevedo-Duncan M, Patel R, Whelan S, Bicaku E. Human glioma PKC-iota and PKC-betaII phosphorylate cyclin-dependent kinase activating kinase during the cell cycle. Cell Prolif. 2002;35:23-36. Pubmedid: 11856176.
  • Bicaku E, Acevedo-Duncan M. Ultrastructural study of protein kinase C-betaII localization during the cell cycle of human glioma cells. Tissue Cell. 2001;33(1):55-62. Pubmedid: 11292171.
  • Yamamoto M, Acevedo-Duncan M, Chalfant CE, Patel NA, Watson JE, Cooper DR. Acute glucose-induced downregulation of PKC-betaII accelerates cultured VSMC proliferation. Am J Physiol Cell Physiol. 2000 Sep;279(3):C587-C595. Pubmedid: 10942708.
  • Acevedo-Duncan M, Pearlman J, Zachariah B. Sensitivity of human glioma U-373MG cells to radiation and the protein kinase C inhibitor, calphostin C. Cell Prolif. 2000;34(1):31-41. Pubmedid: 11284917.
  • Bai W, Oliveros-Saunders B, Wang Q, Acevedo-Duncan M, Nicosia S. Estrogen stimulation of ovarian surface epithelial cell proliferation. In Vitro Cell Dev Biol Anim. 2000;36(10):657-666. Pubmedid: 11229598.
  • Cooper DR, Watson JE, Patel N, Illingworth P, Acevedo-Duncan M, Goodnight J, Chalfant CE, Mischak H. Ectopic expression of protein kinase CbetaII, -delta, and -epsilon, but not -betaI or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells. Arch Biochem Biophys. 1999 Dec;372(1):69-79. Pubmedid: 10562418.
  • Yamamoto M, Acevedo-Duncan M, Chalfant C, Patel N, Watson J, Cooper D. The roles of protein kinase C beta I and beta II in vascular smooth muscle cell proliferation. Exp Cell Res. 1998 May;240(2):349-358. Pubmedid: 9597008.
  • Acevedo-Duncan M, Zhang R, Cooper DR, Greenberg HM. Effects of interferon and PKC modulators on human glioma protein kinase C, cell proliferation, and cell cycle. Neurochem Res. 1997 Jul;22(7):775-784. Pubmedid: 9232628.
  • Acevedo-Duncan M, Collins J, Zhang R, Haller E, Chalfant CE, Cooper DR. In situ effects of interferon on human glioma protein kinase C-alpha and -beta ultrastructural localization. Cell Growth Differ. 1995 Nov;6(11):1353-1365. Pubmedid: 8562473.
  • Acevedo-Duncan M, Zhang R. Heterogeneity of protein kinase C activity in human U-373 and G-26 mice glioma cells. Biochem Biophys Res Commun. 1994;205(1):127-134.
  • Acevedo-Duncan M, Cooper DR, Standaert ML, Farese RV. Immunological evidence that insulin activates protein kinase C in BC3H-1 myocytes. FEBS Lett. 1989 Feb;244(1):174-176. Pubmedid: 2647516.
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